dPCR Influenza and SARS-CoV-2 Wastewater Surveillance Assay Kit for the BioRad QX200™ Droplet Digital™ PCR System

(Cat# 100330) The dPCR Influenza and SARS-CoV-2 Wastewater Surveillance Assay Kit for the Bio-Rad QX200™ Droplet Digital™ PCR System comprises a molecular reagent kit containing all primers, probes, and controls for wastewater surveillance of Influenza A/B and SARS-CoV-2. Assay solutions and appropriate controls are provided for the quantification of 1) Influenza A; 2) Influenza B; 3) SARS-CoV-2; 4) Bovine Coronavirus (BCoV), a matrix recovery control (also called a process control); and 5) Pepper Mild Mottle virus (PMMoV), a fecal indicator that can estimate the human fecal content in a sample. Importantly, the chosen SARS-CoV-2 target differs from 2019- nCoV_N1 to circumvent potential issues with accumulated mutations observed within this region. Through the use of this kit in conjunction with the Bio-Rad QX200™ Droplet Digital™ PCR System, users can monitor Influenza and SARS-CoV-2 levels and normalize for changes in viral recovery caused by the variable and complex nature of wastewater, as well as normalize for human waste input, which can change over time due to dilution and variations in human behavior (travel, tourism, etc.). Contains enough material for 200 samples.