GT-Digital Microbial Source Tracking Human;E. coli; Cow; Pig; Dog; Sketa Panel v1.0 for the Bio-Rad QX600™ Droplet Digital™ PCR System

(Cat# 100980)

GT-Digital Microbial Source Tracking (MST) Human;E. coli;Cow;Pig;Dog;Sketa Panel v1.0 for the Bio-Rad QX600™ Droplet Digital™ PCR System is a molecular reagent kit consisting of all primers, probes, and controls necessary for detection of microbial DNA specific to human, cow, pig and dog Bacteroides species, as well as E. coli species. This assay also utilizes Sketa (Sketa-22, Salmon Sperm DNA) as a matrix recovery control (also called an internal process control), allowing the user to normalize extraction processes for potential differences in DNA recovery. This assay allows identification of fecal contamination sources in freshwater environments, including lakes, rivers, reservoirs, groundwater, and stormwater. The assay targets the HF183 marker for human-associated Bacteroides, CowM2 for cow-associated Bacteroides, Pig2Bac marker for pig- associated Bacteroides, BacCan marker for dog associated Bacteroides, and EC23S marker for E. coli (7-copy gene), located either on the 16S or 23S rRNA genes. Assay solutions and appropriate controls are provided to ensure accurate quantification of all targets. When used in conjunction with the Bio-Rad QX600™ Digital PCR System, this kit facilitates reliable microbial source tracking, supporting environmental water (EW) quality assessments and public health surveillance efforts. Contains enough material for 200 reactions.

Important: MST Human;E. coli;Cow;Pig;Dog;Sketa Panel has been validated on environmental water. This panel can work on wastewater, however further optimization and validation is required by the user.