Applied Biosystems™ QuantStudio™ Absolute Q™ Digital PCR System
Highly sensitive and multiplexed digital PCR Assays for cancer and wastewater applications on the QuantStudio™ Absolute Q™ Digital PCR System. Our extensive experience and patented and patent pending technology mean our assays work well where others may not. This includes samples with very low concentration and high inhibition such as those found in biological samples and wastewater.
Applied Biosystesm™, QuantStudio™ and Absolute Q™ are trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. For Research Use Only. Not for use in diagnostic procedures.
Learn more about the QuantStudio™ Absolute Q™ Digital PCR System at ThermoFisher.com.
GT-Digital Assay Kits for the QuantStudio™ Absolute Q™ Digital PCR System
Complete assay kits include all primers, probes, and controls to measure multiple targets in one reaction well using our highly multiplexed assays.
Wastewater Testing
GT-Digital Wastewater Surveillance Respiratory Pathogen Assay Kit – Contact us
Cancer Research Assays
GT-Digital IDH1/IDH2/NPM1 Mutation Panel
GT-Digital KRAS G12/13 Mutation Detection Assay Kit
GT-Digital ESR1 Mutation Detection Assay Kit – Contact us
Key Benefits
Detect as few as 3 copies of viral RNA per reaction and identify viruses in samples with low viral load
Leverage our GT-Plex™ technology to amplify more targets in each assay, saving you time and money
Assay your samples with validated kits that include all necessary primers, probes, and controls
Eliminate interference from PCR inhibitors using dPCR technology
Targets Available for the QuantStudio™ Absolute Q™ dPCR System
Respiratory Pathogens
SARS-CoV-2, Influenza A, Influenza B, Respiratory syncytial virus (RSV) A/B
Cancer Targets (RUO)
IDH1/IDH2/NPM1 : Detection of IDH1-R132C/H, IDH2-R140Q, IDH2-R172K, and over 30 types of NPM1 insertion mutations.
KRAS G12/G13 : Detect eight mutations in the human KRAS gene within in a single well.
ESR1 : Detect eleven of the most common mutations in the human ESR1 gene.
Custom Development
Don’t see the target(s) you are looking for? We may have just what you need already in development, easily transferred onto the QuantStudio™ Absolute Q™ dPCR System, or quickly developed using our deep experience and patents and patent-pending technology. We are here for you every step of the way to make your testing as easy as possible. Contact us to learn more.
Request the Brochure
Available Assays for the QuantStudio™ Absolute Q™ dPCR System
The GT-Digital IDH1/IDH2/NPM1 Mutation Detection Assay Kit for the QuantStudio™ Absolute Q™ Digital PCR System is a Research-Use Only (RUO), patent-pending molecular reagent kit containing all primers, probes, and controls for the detection and quantification of common mutations found in the human IDH1, IDH2, and NPM1 genes. This multi-target kit contains one assay solution and two qualitative controls. The assay solution contains a TAMRA probe that targets wild-type sequence at IDH1-R132 and Cy5 probes that target IDH1-R132C, IDH1-R132H, IDH2-R140Q, and IDH2-R172K mutations. The assay solution additionally contains a FAM probe which targets wild-type sequence at NPM1-W288 where common insertions occur and a HEX probe targeting wild-type sequence within the same amplicon. The presence of a short nucleotide insertion in the region of the FAM binding sequence results in a HEX-only positive signal (from the absence of FAM signal), whereas the presence of non-mutated NPM1 results in a FAM+HEX double positive signal. The assay was validated for the detection of the Type A NPM1 insertion (c.863insTCTG) and may also detect, but not discriminate between, Type B (c.863insCATG), D (c.863insCCTG), and other insertions occurring between c.863 and c.866. Rare NPM1 insertion types occurring between c.867 to c.875 may also be detected with the assay, resulting in a FAM-only positive signal (from the loss of HEX signal). However, these insertion types have not been tested. Qualitative controls included in the kit contain a mutated control at 10% variant allele frequency (VAF) and a wild-type control at 0% VAF for all target regions.
.
The GT-Digital KRAS G12-G13 Mutation Detection Assay Kit for the QuantStudio™ Absolute Q™ Digital PCR System is a Research-Use Only (RUO), patent-pending molecular reagent kit containing all primers, probes, and controls for the detection and quantification of common mutations found in the human KRAS gene. This multi-target kit contains one assay solution and two qualitative controls. The assay solution targets the KRAS G12C, G12V, and G12A mutations in FAM; the G12S, G12R, and G13C mutations in HEX; the G12D and G13D mutations in Cy5; and wild-type KRAS G12-G13 in ABY. A 10% mixed mutant (MT) control and wild-type (WT) qualitative control are included in the kit. Contains enough material for 50 reactions.
Quantify Your Target in 3 Easy Steps
Receive kit via overnight shipping within five business days
Extract nucleic acid from your sample using your lab’s preferred method
Perform the PCR assay on your sample using your instrument of choice
GT Molecular PCR Kit Advantages
FAQs
Short on Time? Let Us Do the Testing for You
