Food and beverage microbial PCR kits — Salmonella and STEC
One enrichment, a 15-minute extraction, and a single multiplex well that reads both pathogens. Set up at the end of the shift, read presumptive results the next morning. No proprietary reader, no per-test license.
- 2 pathogens – 1 test
- 15-min extraction
- Next-morning results
- AOAC validation in progress
Runs on the Bio-Rad CFX96™, Thermo Fisher QuantStudio™ 5 and Agilent AriaMx™ · All controls included · Research Use Only
One enrichment. One Well. Both Pathogens.
Featured Kit – Cat# 100640
Many labs run Salmonella and STEC as separate enrichments and separate assays. The GT Real-Time Salmonella & STEC Detection Assay reads both from a single enrichment in a single multiplex well, which halves the reactions, master mix and plate space per sample.
STEC is called on two independent gene targets, stx and eae.
Set up in the evening. Read results in the morning.
Add your sample to Buffered Peptone Water and incubate 16–24 h at 37 °C.
A simple 15-minute DNA prep for up to 96 samples and run Real-Time PCR multiplex assay with GTM qPCR Master Mix.
Presumptive Salmonella and STEC calls from one well, with the extraction control in every reaction.
Simplify your testing.
Key Benefits
Identify pathogens with single-cell sensitivity after enrichment
Leverage our specialized assay designs to amplify more targets in each assay, saving you time and money
Assay your samples with complete kits that include all necessary primers, probes, and controls
Detect common microbes associated with Food & Beverage regulation using our robust, easy-to-use Real-Time PCR (RT-PCR) kits
In AOAC Performance Tested Method validation
What’s being validated
- Program – AOAC Performance Tested Method (PTM)
- Standards – SMPR 2020.002 (Salmonella) · SMPR 2020.012 (STEC)
- Reference – FDA-BAM Ch. 5 (Salmonella) and Ch. 4A (STEC)
- Inclusivity – ≥100 Salmonella strains across required subspecies; STEC panel total of 50 strains, including the Top 7 STEC and at least 5 additional serogroups
- Exclusivity – 30 non-target organisms per target
- Food matrix – Leafy greens (raw spinach, up to 375 g). Testing another matrix? Ask us for data and validation support.
Status: Validation in progress. Contact us to learn more.
Food & Beverage Testing Microbial Targets
Salmonella and STEC
Detect Salmonella and Shiga toxin-producing E. coli simultaneously using our multiplexed RT-PCR kit. Assay targets genomic loci specific to Salmonella and Shiga toxin-producing E. coli (STEC) in a single reaction for leafy greens (raw spinach).
Salmonella
Detect pathogenic Salmonella species from the following food products:
- raw chicken breasts
- raw ground turkey
- chicken carcass rinse
- deli turkey
- plastic and stainless-steel surface swabs
More assays for the same bench. Ask about your sample matrix!
FAQs
