Flu A Sequencing Kits for Wastewater

The industry’s first commercially developed and validated Influenza A NGS offering for wastewater. Choose between our all-inclusive Sequencing Service or our high-sensitivity NGS Library Prep Kit.

* Research Use Only. Not intended for diagnostic procedures.

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Industry First Offerings

Wastewater Influenza A Variant Tracking

GT Molecular provides the first commercially available Flu A NGS workflow optimized specifically for the fragile RNA found in wastewater matrices. Stop relying on “homebrew” protocols, use the validated standard.

Our Flu A Sequencing service & GT Fast Prep NGS kits are designed to provide target amplicon sequencing of most important subtypes (H1N1 and H3N2) and provide actionable information for flu variant spread.

Gain precision surveillance for a rapidly evolving virus, from sample to sequencing to bioinformatic reports.

Sequencing Service NGS Library Prep Kit

Key Benefits

What sets us apart? Unlike standard testing, our end-to-end workflow is optimized specifically for the complex matrix of wastewater.

Streamlined

All-in-one sample collection kit and multiplexed, automation-ready kit with minimal hands on-time and rapid results

High sensitivity

Optimized and validated for low-titer samples from wastewater, a known difficult matrix

Mutant tracking

Monitor antigenic drift and identify markers associated with vaccine resistance for H1N1 and H3N2 subtypes

Rapid insights

Minimal hands-on time, easy-to-interpret bioinformatics reports included with the testing service & as an add-on service with the NGS library prep kit.

Case Study: Flu A NGS Sequencing

Sequencing influenza A in wastewater provides community-level insight into circulating strains and supports continuous, long-term monitoring.

For the first time, actionable influenza sequencing data can be generated from wastewater, a matrix where flu RNA is notoriously fragile and difficult to detect.

This approach answers key public health questions: Is influenza A present? Which strains are circulating? What are their relative fractions? How are those strains changing over time?

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Flu A Sequencing Bioinformatics Report

A comprehensive, easy-to-interpret bioinformatics report provides the following:

Identified strains
Relative abundance
Confidence metrics
Interpretation summaries
Ability to export data to sheets

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Gold Standard NGS Bioinformatics Service

Optimized pipeline squeezes out every bit of insight present in your data from wastewater, a notoriously difficult matrix.

High-performance Cloud Computing with thorough robustness checks using synthetic and lab data.

Dedicated experts in Bioinformatics with extensive domain knowledge

Continuous investment to maintain up-to-date tools

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Most Requested NGS Offerings

GT-Fast Prep Influenza A Library Kit for Illumina®

The GT-Fast Prep Influenza A Library Kit for Illumina® is a molecular reagent kit containing all primers and reagents for the amplification and downstream library preparation of influenza A gRNA extracts from wastewater matrices.

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Flu A Sequencing Service Add-on for Wastewater

(Cat# 200269) This testing service covers Influenza A NGS testing that includes sequencing preparation, NGS H1 H3 Flu A Sequencing for segments H and N, data analysis, and consolidated report. This add-on testing can be applied to any sample received by our laboratory that has already been quantified using our digital PCR (dPCR) testing service for the […]

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GT-Fast Prep ARTIC SARS-CoV-2 Library Preparation Kit

The GT-Fast Prep ARTIC SARS-CoV-2 Library Preparation Kit is a molecular reagent kit containing all primers and reagents for the amplification and downstream preparation of SARS-CoV-2 gRNA extracts from wastewater matrices.

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FAQs

What makes your Flu A Sequencing offerings so special?

This is the industry’s first commercially developed and validated Influenza A NGS offering for wastewater. Wastewater is a notoriously difficult sample matrix. GT Molecular has years of experience in developing and optimizing assays to perform above and beyond in wastewater. We provide results where other offerings may miss. Combined with our exemplary quality control metrics, bioinformatics pipeline, and customer service, and you can see why our customers choose us time and time again.

Like our SARS-CoV-2 Next-Generation Sequencing Service and Library Preparation Kit, we have significantly decreased both hands-on-time and time to results.

How do you ensure viable samples for flu sequencing?

It is recommended to run digital PCR (dPCR) on wastewater samples to ensure presence of Flu A prior to sequencing.

GT-Fast Prep Influenza A Library Kit for Illumina: Recommended dPCR kits to use as a quality control metric for sequencing include the GT-Digital Flu;SC2;RSV Wastewater Surveillance Panel (Cat# 100735 or 100740) to identify and quantify positive influenza A from wastewater extracts.

Testing Service: We perform dPCR testing as part of our testing service as a quality control metric for our Flu A Sequencing Service. These tests are also available as a standalone testing service for regular monitoring and quantification of Respiratory Pathogens in wastewater.

What is included in your Flu A offerings?

The GT-Fast Prep Influenza A Library Kit for Illumina consists of primers and reagents required for identifying known and/or novel variants of the influenza A virus from wastewater. Each kit comes with materials sufficient for amplification and multiplexing of up to with sufficient overage to enable full workflow automation. Reagents needed for nucleic acid extraction from wastewater, library QC, and for operating an Illumina sequencer are not included.

Our newly released GT-EZ Bind™ accelerates NGS library preparation with efficient bead-based purification, delivering clean, size-selected DNA for high-quality sequencing data.

Our Flu A NGS Testing Service includes sample kits and shipping, nucleic acid extraction, dPCR screening for Flu A positive samples, sequencing preparation, NGS H1 H3 Flu A sequencing for segments H and N, data analysis, and consolidated report.

How soon will I receive results?

The GT-Fast Prep Influenza A Library Kit for Illumina Get sequencing results in as little as 3.5 hours of hands on time and go from sample to sequencing ready library in just 8 hours.

Testing Service: The turnaround time is 10 business days from sample receipt, with sequencing results and sample information compiled into a single consolidated report.

Does NGS replace dPCR?

No, the two methods complement each other. dPCR confirms the presence and quantification of Flu A, then NGS provides strain detail. Our kits and testing methods are designed and optimized for wastewater surveillance.

Can this service detect new strains?

Yes, within coverage and depth limits.

Can you analyze my FastQ files?

Yes! We offer the first ready-made bioinformatics solution for flu analysis in wastewater. Because standard clinical pipelines aren’t designed for the mixed-sample complexity of wastewater, this service delivers the specialized reporting your lab needs, right out of the box. Contact us for pricing and access to an example, interactive report.

GT Molecular offers affordable FastQ analysis with a formatted, easy to read PDF report.