product detail

GT Real-Time Salmonella Detection Kit for Food & Beverage Testing

SKU: 101155
Contact to Order

Description

(Cat# 101155) – For a package kit that includes extraction kit, contact us.

This assay kit provides users with an end-to-end workflow to detect pathogenic Salmonella species from the following food products: raw chicken breasts, raw ground turkey, chicken carcass rinse, deli turkey, and stainless-steel surface swabs. The GT-EZ Extraction Kit (sold separately, contact us for packaged kit options) comprises a Research Use Only (RUO) reagent kit containing all reagents necessary for the extraction of bacterial DNA from food matrices. The GT Real-Time Salmonella Detection Assay Kit comprises a RUO molecular reagent kit containing all primers, probes, master mix, and controls necessary for the detection of genomic DNA from pathogenic Salmonella­­. The assay kit is validated for use with the GTM qPCR Master Mix (sold separately, contact us for packaged kit options).

Contents

The GT-EZ Extraction Kit contains two extraction buffers sufficient for 96 preparations. The GT Real-Time Salmonella Detection Assay Kit contains one ready-to-use Master Mix, one pre-mixed primer-probe solution sufficient for 96 x 20 μL reactions and two positive control solutions.

Validated Food Matrices

This kit has been validated for use with the following cannabis and food materials in Buffered Peptone Water (BPW):

  • Chicken Carcass Rinses (30 mL in 30 mL BPW)
  • Raw Chicken Breasts (Up to 375 g in up to 1,125 mL BPW – 1:4 dilution)
  • Raw Ground Turkey (Up to 375 g in up to 1,125 mL BPW- 1:4 dilution)
  • Deli Turkey (Up to 375 g in up to 1,125 mL BPW – 1:4 dilution)
  • Stainless Steel (1 sponge in 50 mL BPW)

For use in other sample types such as pre-sliced cantaloupe, cheddar cheese block, or deli-style cooked pre-sliced turkey contact us.

Equipment

The GT Real-Time Salmonella Detection Assay Kit is validated for use with the Agilent AriaMx™, Thermo Fisher QuantStudio™ 5, and Bio-Rad CFX96™ Real-Time PCR Systems. Other systems capable of reading FAM, SUN/HEX, Texas Red/ROX, and Cy5 fluorophores are likely compatible but may require further optimization.

The workflow consists of four steps, sample enrichment, DNA extraction, Real-Time PCR, and analysis. For questions or recommendations regarding the workflow below, please contact us.