QIAGEN QIAcuity Kits

Platform Highlights

About the QIAGEN QIAcuity

The QIAGEN QIAcuity is an integrated, nanoplate-based digital PCR (dPCR) system that delivers absolute quantification in approximately two hours. By eliminating separate droplet generation, thermal cycling, and manual reading, it offers a streamlined, walk-away workflow.

  • Integrated Automation: Replaces multi-step droplet workflows with a single, fully automated nanoplate system.

  • Flexible Throughput: Available in three tiers (QIAcuity One, Four, and Eight) to match focused or large-scale projects.

  • Multi-Color Multiplexing: Supports up to 5 detection channels (including FAM, HEX/VIC, ROX/TexasRed, and Cy5) depending on the model.

  • Absolute Quantification: Uses Poisson statistics to count target molecules directly, eliminating standard-curve variability for better cross-lab reproducibility.

  • Platform-Validated Kits: GT Molecular kits are tuned specifically for QIAcuity’s partitioning chemistry and channel assignments with primers, probes, and controls including matrix-recovery controls.

  • Target Applications: GT Molecular’s assays are purpose-built for this platform for wastewater surveillance, microbial source tracking, AMR monitoring, oncology, and cell/gene therapy.

For research use only. Not for use in diagnostic procedures.
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Key Benefits

Sensitive

Detect as few as 3 copies per reaction. Resolve rare alleles and copy-number differences where qPCR typically struggles.

Multiplexed

Leverage our GT-Plex™ technology to amplify multiple targets in a single nanoplate well, saving you time, reagents, and sample volume.

Easy to Use

Validated kits ship with all primers, probes, and controls. Drop-in compatibility with the QIAcuity nanoplate workflow — no protocol tinkering required.

Robust

Eliminate interference from PCR inhibitors using dPCR endpoint detection. Reliable performance on wastewater, plasma, FFPE, transduced cells, and other complex matrices.

Targets Available for the QIAGEN QIAcuity digital PCR Platform

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Digital illustration of lungs with virus particles representing respiratory infections.

Respiratory Pathogens

Key singleplex, duplex, and multiplex panels for respiratory diseases impacting communities on your QIAcuity nanoplate platform.

  • SARS-CoV-2 & variants
  • Influenza A & B and H5 subtypes
  • Respiratory syncytial virus (RSV) A & B
  • Measles (MeV)
  • Tuberculosis (TB)
  • Bordetella pertussis (Whooping cough)
  • Human metapneumovirus (HMPV)
  • Enterovirus D68 (EV-D68)
Respiratory Panels for QIAcuity
Antimicrobial resistance Microbial culture in petri dish for laboratory testing and research.

Antimicrobial Resistance Targets

Monitor for the presence of major antimicrobial resistance (AMR) genes circulating. Available in duplex assays. Interested in higher order multiplexing? Let us know!

  • Colistin (mcr-1)
  • Integrase 1 (intI1)
  • Tetracycline (tetW)
  • Vancomycin (vanA)
  • Carbapenem (NDM, IMP, OXA-48, VIM, KPC)
  • Beta-Lactam (blaCTX, BlaTEM, blaCMY-2, blaSHV)
AMR Panels for QIAcuity
High-resolution image of bacteria cells for microbiology and biotech applications.

Parasite, Blood, Fungal & Enteric Pathogens

Detect a wide array of high-priority viral, bacterial, fungal, and parasitic pathogens. By screening for these targets understand outbreak response and public health surveillance.

  • Candida auris
  • Campylobacter spp.
  • Cyclospora cayetanensis
  • Human immunodeficiency virus (HIV)
  • Hepatitis A (HepA or HAV)
  • Orthopox viruses – Non-variola orthopox (NVO), Mpox virus (MPXV) Clade I and Clade II
  • West Nile virus (WNV)
  • Dengue virus (DENV)
  • Zika virus (ZIKV)
  • Chikungunya (CHIKV)
  • Norovirus GI & GII
  • Adenovirus
Blood & Enteric Panels for QIAcuity
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Microbial Source Tracking (MST)

Use molecular profiling to trace bacteria back to their specific host organisms by targeting unique genetic differences. By pinpointing the exact sources of fecal contamination within a watershed or surface water body, MST delivers the actionable insights needed to address pollution.

  • E. coli (EC23S target)
  • Enterococcus spp. (Entero1a)*
  • Human (HF183)
  • Cow (CowM2)
  • Pig (Pig2Bac)*
  • Gull (Gull4)
  • Dog (BacCan)
  • Canadian Goose (CGOF1)
  • Goose (ND2)
  • Ruminants (Rum2Bac)*
  • Avian (GFD)*
  • Bacteroides (GenBac3)*

*Contact us for interest in available dPCR assay compatibility on the QIAcuity

Microbial Source Tracking Panels
Cancer cells, Blood cells and immune components in a detailed microscopic view.

Cancer Research Assays

Use sensitive digital PCR to precisely detect and quantify critical oncogenic biomarkers. By targeting key mutations and fusion genes, these RUO kits deliver the high-resolution genetic insights needed to track disease progression and therapeutic resistance in cancer research.

Interested in more panels? Let us know. Research use only. Not intended for diagnostic procedures.

Oncology (RUO) Panels
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Section: Cell & Gene Therapy QC

For cell and gene therapy programs running on the QIAGEN QIAcuity, digital PCR has become the standard for vector copy number determination, AAV titer measurement, transgene quantification, and residual plasmid detection — applications where the QIAcuity’s nanoplate automation, audit-friendly software, and absolute-quantification chemistry make GMP-aligned workflows simpler to validate. dPCR is widely considered the gold standard for AAV viral titer because it removes standard-curve variability from cross-batch comparisons.

GT-Digital™ Vector Copy Number (WPRE) Assay Kit for the QIAcuity

GT Molecular’s VCN WPRE kit detects the woodchuck hepatitis post-transcriptional regulatory element (WPRE), a universal sequence in many lentiviral and AAV vector backbones, enabling precise integrated vector copy number quantification in transduced cells. Validated on the QIAcuity nanoplate workflow for gene therapy product release, process development, and bioprocess monitoring. Research use only.

Vector Copy Number Assays
Pipetting samples for GT Molecular assays and testing services

Don't see your targets here?

Let us know!

Contact us to see what assays are in development, partnership options, or to learn more about our custom assay design service. GT Molecular works directly with academia, government, industry, and CGT/bioprocessing partners to build the highest quality QIAcuity assays possible.

Get industry leading performance

GT Molecular’s patented and patent-pending technology, combined with our proprietary bioinformatics pipeline, delivers the precision and reproducibility QIAcuity owners need for production-scale surveillance and regulated workflows.

Complete, validated assay kits

Our assay kits are optimized to provide reliable results validated for your sample type, complete with all primers, probes, and controls. See why our customers choose us time and time again.

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Popular Assays for QIAcuity dPCR Platforms

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Vector copy number (VCN) WPRE digital PCR kit for the QIAGEN QIAcuity — gene therapy QC
(Cat# 101015) GT-Digital™ Vector Copy Number: WPRE Assay Kit v1.0 for the QIAGEN QIAcuity® Digital PCR System is a molecular reagent kit consisting of all primers, probes, and controls necessary for the detection and quantification of a common viral vector genetic element (WPRE), present in lentiviral, adeno-associated, and retroviral vectors. It also detects a housekeeping […]
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GT-Digital™ HIV Wastewater Surveillance Assay v1.0 for the QIAGEN QIAcuity® Digital PCR System
The GT-Digital™ TB Wastewater Surveillance Assay v1.0 for the QIAGEN QIAcuity® Digital PCR System comprises a molecular reagent kit containing all primers, probes, and controls required for the wastewater surveillance and quantification of the Mycobacterium tuberculosis Complex (MTBC), including Mycobacterium tuberculosis (Mtb).
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See all available QIAcuity dPCR kits
"GT Molecular has had the most informative and thought-out instructions that have been included with the assay kits we have tried."
Christine Laba, Oakland University

Quantify Your Target in 3 Easy Steps

1
Receive

Receive kit via overnight shipping within five business days

2
Process

Extract nucleic acid from your sample using your lab’s preferred method

3
Assay

Load the QIAcuity nanoplate and run on your QIAcuity

GT Molecular PCR Kit Advantages

Validated
Our dPCR kits are pre-measured, tested, and validated to minimize troubleshooting and downtime
Efficient
Multiplexing several targets in each assay saves you time and money
Complete
Every PCR kit includes all of the primers, probes, and controls required for your assay

FAQs

Don't have a QIAcuity? GT Molecular's national testing lab uses the same assays!

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Influenza A/B Wastewater Test Testing Service
(Cat# 100315) Wastewater Testing Service targets Influenza A and Influenza B. Includes collection kit and Priority Overnight return shipping.
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Flu A Sequencing Service Add-on for Wastewater
(Cat# 200269) This testing service covers Influenza A NGS testing that includes sequencing preparation, NGS H1 H3 Flu A Sequencing for segments H and N, data analysis, and consolidated report. ​ This add-on testing can be applied to any sample received by our laboratory that has already been quantified using our digital PCR (dPCR) testing service for the […]
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Microbial Source Tracking Testing Service
This testing service covers microbial source tracking testing that includes digital PCR (dPCR) detection and absolute quantification for sources of fecal contamination. For sequencing we offer our 16S sequencing service for microbial community analysis (SKU: 200258).  Bacteria found in different host organisms are identified by targeting known gene differences. This testing service can be used […]
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