The GT-Digital ESR1 Mutation Panel for the Absolute Q™ Digital PCR System is a Research-Use Only (RUO), patent-pending molecular reagent kit containing all primers, probes, and controls for the detection and quantification of common mutations found in the human ESR1 gene.
GT Molecular is thrilled to introduce four new assays for the Applied Biosystems™ QuantStudio™ Absolute Q™ Digital PCR System. We are bringing our deep experience in dPCR, and patented and patent pending, ultra-sensitive dPCR assay kits to the Absolute Q™ system.
The GT-Digital™ IDH1/IDH2/NPM1 Mutation Detection Assay Kit for the QuantStudio™ Absolute Q™ Digital PCR System is a Research-Use Only (RUO), patent-pending molecular reagent kit containing all primers, probes, and controls for the detection and quantification of common mutations found in the human IDH1, IDH2, and NPM1 genes. This multi-target kit contains one assay solution and two qualitative controls. The assay solution contains a TAMRA probe that targets wild-type sequence at IDH1-R132 and Cy5 probes that target IDH1-R132C, IDH1-R132H, IDH2-R140Q, and IDH2-R172K mutations. The assay solution additionally contains a FAM probe which targets wild-type sequence at NPM1-W288 where common insertions occur and a HEX probe targeting wild-type sequence within the same amplicon. The presence of a short nucleotide insertion in the region of the FAM binding sequence results in a HEX-only positive signal (from the absence of FAM signal), whereas the presence of non-mutated NPM1 results in a FAM+HEX double positive signal. The assay was validated for the detection of the Type A NPM1 insertion (c.863insTCTG) and may also detect, but not discriminate between, Type B (c.863insCATG), D (c.863insCCTG), and other insertions occurring between c.863 and c.866. Rare NPM1 insertion types occurring between c.867 to c.875 may also be detected with the assay, resulting in a FAM-only positive signal (from the loss of HEX signal). However, these insertion types have not been tested. Qualitative controls included in the kit contain a mutated control at 10% variant allele frequency (VAF) and a wild-type control at 0% VAF for all target regions.
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The GT-Digital™ KRAS G12-G13 Mutation Detection Assay Kit for the QuantStudio™ Absolute Q™ Digital PCR System is a Research-Use Only (RUO), patent-pending molecular reagent kit containing all primers, probes, and controls for the detection and quantification of common mutations found in the human KRAS gene. This multi-target kit contains one assay solution and two qualitative controls. The assay solution targets the KRAS G12C, G12V, and G12A mutations in FAM; the G12S, G12R, and G13C mutations in HEX; the G12D and G13D mutations in Cy5; and wild-type KRAS G12-G13 in ABY. A 10% mixed mutant (MT) control and wild-type (WT) qualitative control are included in the kit. Contains enough material for 50 reactions.
(Cat# 100785)
The GT-Digital™ Flu;SC2;RSV Wastewater Surveillance Panel for the QuantStudio? Absolute Q™ Digital PCR System comprises a molecular reagent kit containing assay solutions and appropriate controls for the detection and quantification of 1) Influenza A; 2) Influenza B; 3) SARS-CoV-2; and 4) Respiratory Syncytial Virus (RSV). Through use of this kit in conjunction with the QuantStudio™ Absolute Q™ Digital PCR System, users can monitor influenza virus, SARS-CoV-2 and RSV levels. Contains enough material for 200 reactions.
The GT-Real Time WNV;DENV;ZIKV Mosquito Surveillance Assay v1.0 comprises a molecular reagent kit containing primers, probes, controls, and the master mix required for the mosquito surveillance of West Nile virus (WNV), Dengue virus (DENV), and Zika virus (ZIKV). This kit also includes an internal process control for the detection of mosquito RNA, mosquito internal process control (MIPC). The use of these kits in conjunction with the Bio-Rad™ CFX Opus™ 96 Real-Time PCR or Thermo Fisher Scientific QuantStudio™ 5 Real-Time PCR Systems allows users to monitor WNV, DENV, and ZIKV in mosquitoes.
The GT Real-Time Microbial Source Tracking Beach Detection Panel v 1.0 comprises a molecular reagent kit containing all primers, probes, controls, and master mix required for the detection of host-associated genetic markers in environmental freshwater samples using Real-Time PCR platforms. The panel enables identification of fecal contamination sources, including human and animal inputs, to support microbial source tracking (MST) investigations in rivers, lakes, reservoirs, groundwater, and stormwater. The assay targets host-associated Bacteroides markers and a process control, including human HF183, gull Gull4, canine BacCan, and goose ND2 markers, as well as an extraction/process control – Sketa (Salmon DNA target).
The GT Real-Time Microbial Source Tracking Inland Detection Panel v 1.0 comprises a molecular reagent kit containing all primers, probes, controls, and master mix required for the detection of host-associated genetic markers in environmental freshwater samples using Real-Time PCR platforms. The panel enables identification of fecal contamination sources, including human and animal inputs, to support microbial source tracking (MST) investigations in rivers, lakes, reservoirs, groundwater, and stormwater. The assay targets host-associated Bacteroides markers and a process control, including human HF183, ruminant Rum2Bac, canine BacCan, and pig Pig2Bac markers, as well as an extraction/process control – Sketa (Salmon DNA target).
This testing service covers microbial source tracking testing that includes digital PCR (dPCR) detection and absolute quantification for sources of fecal contamination. For sequencing we offer our 16S sequencing service for microbial community analysis (SKU: 200258). Bacteria found in different host organisms are identified by targeting known gene differences. This testing service can be used […]
